Bayer cross logo sml

Help us design an assay to quantify multiple proteins in a single sample

It is a routine practice to measure the amount of a protein in biological samples using Enzyme-Linked ImmunoSorbent Assay (ELISA). A sandwich ELISA is based on two non-competing antibodies that bind to different regions of the same protein. Currently, one protein is measured in one protein-specific assay.

13K
top 5
28
submissions
DONE
13 months ago
Deliverables

We are seeking technology and/or instrumentation that will enable us to quantify multiple proteins simultaneously in a single sample. Please include:
1) A description of your method
2) Supporting literature and examples if possible

Criteria:
The assay does not have to be limited to traditional immunoassay methods. Special consideration will be given to ideas that are different than existing platforms such as Luminex and Meso Scale Discovery.

The assay MUST have but is not limited to the following qualities:
- Ability to quantify a minimum of 3-4 proteins in a single sample,
- High throughput (>150 samples per day) and suitable for use with liquid handler automation,
- Simple lab-to-lab transfer,
- Low maintenance and cost for equipment and reagents.

The assay must NOT have the following qualities:
- Lower sensitivity than ELISA

Important: Please focus on proposing a unique, original, and novel solution. Avoid techniques that are already in practice if possible.

Submit your solution
Leaderboard
This challenge has received 28 submissions
Top 5 share 13K
2500 Square pic img 1139 University of Texas at Austin
2500 K Bowdoin College
2500 Square pic 3295460 University of Toronto
2500 Square pic snapchat  2171327898797258840 Oregon State University
2500 Square pic ryan murphy 2 Purdue University
Contact Us!
We would love for you to write on our MindSumo college blog: Details here